Gill Center for Biomolecular Science

Phone:

812-856-2684

Email:

jm99@indiana.edu

Department:

Psychological and Brain Sciences

Campus:

IU Bloomington

Education:

• Ph.D., U.C. San Diego, 2001
• B.S., University of Washington, 1997
• B.A., University of Washington, 1988

Research interests:

The central goal of my research has been to characterize cannabinoid signaling in the brain and the eye. Cannabinoids have been with us for thousands of years but only recently have we begun to learn how they work in the body. Cannabinoid CB1 receptors are nearly ubiquitous in the CNS and are involved in many important functions including (un-)learning and memory, pain, and motor control. The cannabinoid signaling system includes several receptors, endocannabinoids, enzymes that produce and break them down. In neuronal studies my primary approach is to use electrophysiology in combination with molecular biology and anatomy to investigate specific mechanisms of cannabinoid signaling. I have also gradually returned to the subject of my graduate work—cannabinoid signaling in the eye—where we have several projects relating to glaucoma, cell migration, and retinal function.

Representative publications:

• Mitjavila J, Yin D, Kulkarni PM, Zanato C, Thakur GA, Ross R, Greig I, Mackie K, Straiker A. Enantiomer-specific positive allosteric modulation of CB1 signaling in autaptic hippocampal neurons. Pharm Res. 2017 Nov 20. pii: S1043-6618(17)30871-X.
• Miller S, Hu S, Leishman E, Morgan D, Wager-Miller J, Bradshaw H, Straiker A. A GPR119 signaling system in the murine eye regulates intraocular pressure in a sex-dependent manner. IOVS, 2017 58(7):2930-2938.
• Dhopeshwarkar A, Murataeva N, Makriyannis A, Straiker A, Mackie M. Two Janus cannabinoids that are both CB2 agonists and CB1 antagonists. J Pharm Exp Therap. J Pharmacol Exp Ther. 2017 Feb;360(2):300-311.
• Miller S, Leishman E, Oehler O, Daily L, Wager-Miller J, Bradshaw H, Straiker A. Evidence for a GPR18 role in diurnal regulation of intraocular pressure. IOVS, 2016 Nov 1;57(14):6419-6426.
• Smith TH, Blume LC, Straiker A, Cox JO, David BG, McVoy JS, Sayers KW, Poklis JL, Abdullah RA, Chen CK, Mackie K, Elphick MR, Howlett AC, Selley DE. Cannabinoid receptor interacting protein 1a (CRIP1a) modulates CB1 receptor signaling and regulation. Mol Pharmacol. In Press.
• Morgan D, Davis B, Kearn C, Marcus D, Cook A, Wager-Miller J, Straiker A, Myoga M, Karduck J, Leishman E, Sim-Selley L, Czyzyk T, Bradshaw H, Selley D, Mackie K. Mutation of putative GRK phosphorylation sites in the cannabinoid receptor 1 (CB1R) confers resistance to cannabinoid tolerance and hypersensitivity to cannabinoids in mice. Journal of Neuroscience. 2014;34(15):5152-63.
• Jain T, Wager-Miller J, Mackie K, Straiker A. DGLα and DGLβ cooperatively regulate the production of 2-arachidonoyl glycerol in autaptic hippocampal neurons. Molecular Pharmacology. 2013 84(2):296-302.
• Straiker A, Min Kyung-Tai, Mackie K. Fmr1 deletion enhances and ultimately desensitizes CB1 signaling in autaptic hippocampal neurons. Neurobiology of Disease. 2013 56:1-5.
• Caldwell M, Hu SSH, Viswanathan S, Bradshaw H, Kelly M, Straiker A. A GPR18-based signaling system regulates IOP in murine eye. British Journal of Pharmacology. 2013 169(4):834-43.
• Atwood B, Straiker A, Mackie K. CB2 cannabinoid receptor expression in autaptic cultured neurons inhibits synaptic transmission. Neuropharmacology. 2012 63(4):514-23.
• Hudson BD, Beazley M, Szczesniak AM, Straiker A, Kelly M. Indirect sympatholytic actions at β-adrenoceptors account for the ocular hypotensive actions of cannabinoid receptor agonists. Journal of Pharmacology and Experimental Therapeutics. 2011 Dec; 339(3):757-67.
• Straiker A, Wager-Miller J, Hutchens J, Mackie K. Differential signaling in human cannabinoid CB1 receptors and their splice variants in autaptic hippocampal neurons. British Journal of Pharmacology. 2012 Apr;165(8):2660-71.
• Straiker A, Wager-Miller J, Mackie K. The CB1 cannabinoid receptor C-terminus regulates receptor desensitization in autaptic hippocampal neurones. British Journal of Pharmacology. 2012 165(8):2652-9.
• Straiker A, Wager-Miller J, Hutchens M, Hu S, Blankman JL, Cravatt BF, Mackie K. COX-2 and FAAH can regulate the time course of depolarization-induced suppression of excitation. British Journal of Pharmacology. 2011 Nov; 164(6):1672-83.
• Atwood B, Lee D, Straiker A, Widlanski TS, Mackie K. CP47,497-C8 and JWH073, commonly found in “spice” herbal blends, are potent and efficacious CB1 cannabinoid receptor agonists.European Journal of Pharmacology. 2011 659(2-3):139-45.