Anthony M. Zador, M.D., Ph.D., from Cold Spring Harbor Laboratory, will be receiving the 2018 Gill Transformative Investigator Award. He will be presenting a lecture at the Symposium entitled: Sequencing the connectome.
Abstract: To study the connectivity of neural circuits at high resolution, we have developed an approach based on DNA sequencing that allows us to determine the connectivity of single neurons. The key idea is to tag each neuron with a random nucleotide sequence (a "barcode"), which can be read out by high-throughput sequencing. By recasting neuroanatomy, which is traditionally viewed as a problem of microscopy, as a problem of sequencing, MAPseq harnesses advances in sequencing to permit high-throughput interrogation of brain circuits. The ability to read out brain circuitry in a high-throughput way has the potential to transform system neuroscience and our understanding of both normal brain function, and the causes underlying neuropsychiatric disorders including autism, schizophrenia, and depression.