Istvan Katona of Indiana University - Bloomington will present a lecture entitled: PharmacoSTORM super-resolution imaging.
Abstract: Pharmacological measurements in tissue preparations have traditionally been performed by using autoradiography. While this approach has the quantitative power, the limited spatial resolution renders cell- or subcellular compartment-specific measurements difficult. Anatomical measurements in tissue preparation have usually been carried out by antibody-based immunostaining. However, there are numerous proteins targets for which no specific antibody is available, and immunostaining has often limited quantitative power compared to autoradiography. In the present talk, I will introduce a novel approach that combines the strengths of autoradiography and immunostaining. By using fluorophore-tagged receptor ligands and enzyme inhibitors together with STORM super-resolution imaging, we could visualize target engagement sites in cells and tissue preparations. I will show the broad applicability of this approach by presenting examples for different protein families and different pharmacological measurements. In addition, I will introduce a workflow for the visualization and quantification of the nanoscale binding sites of an FDA-approved and in vivo applied medicine in a cell-type- and compartment-specific manner within a complex brain circuit.