Abstract: Palmitoylation is the most common post-translational lipid modification in the brain and is exceedingly important in the regulation of protein stability, trafficking and functioning. Approximately 41% of all identified synaptic proteins are substrates for palmitoylation, and the differential palmitoylation of synaptic substrates has been reported in response to synaptic activity underscoring the potential role for palmitoylation in the regulation of synapse formation and plasticity. Our lab has shown that zDHHC9, one of the 23 enzymes that mediate palmitoylation, is essential for inhibitory synapse formation and proper myelination. Loss of zDHHC9 in mice can lead to seizure -like activity similar to that observed in patients with zDHHC9 nonsense mutations. Using proteomic analysis, we have identified a list of synaptic proteins that are differentially palmitoylated in the hippocampus of mice that have undergone fear conditioning, and demonstrate that differential palmitoylation of synaptic proteins is critical for synapse plasticity.
Regulation of Synapse Formation and Plasticity by Palmitoylating Enzymes by Shernaz Bamji, Ph.D.
Friday, October 09, 2020
12:00 PM – 1:00 PM